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. 2023 Oct 27;22:293. doi: 10.1186/s12933-023-02025-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Klotho exhibited the potential to alleviate podocyte injury aggravated by renal ox-LDL deposition in DKD. A, B IF staining was conducted using WT1 and Nephrin to evaluate the expression of Klotho on podocytes in the kidneys of DKD patients and mice, in comparison to HC and WT. C Serum Klotho levels in normal and DKD mice were detected by ELISA assay. D Additionally, IF analysis using WT1 and Nephrin was carried out to assess the expression of Klotho in podocytes of WT DKD, KL^+/− DKD and TgKL DKD, respectively. E Furthermore, IF analysis using WT1 and Nephrin was performed to evaluate the effect of Klotho on the deposition of ox-LDL in podocytes of the three groups, respectively. F Representative western blot and summarized data showing the effects of Klotho on the relative protein levels of Podocin and apoptosis-associated, Bax in podocytes stimulated with HG and ox-LDL. G Immunofluorescence staining analysis of Klotho highlighting its capacity to mitigate ox-LDL deposition and podocyte injury induced by co-stimulation with HG and ox-LDL. ns no significant; *P < 0.05; **P < 0.01; ***P < 0.001