TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genetic marker(s) | Reference or source |
|---|---|---|
| Strains | ||
| Acinetobacter sp. strain ADP1 (=BD413) | Wild type | 13 |
| Acinetobacter sp. strain WH364 | alk | 7 |
| Acinetobacter sp. strain WH405 | alkM::lacZ, Kmr | This study |
| Acinetobacter sp. strain WH406 | alkR(ΔMluI-StyI), Cmr | This study |
| Acinetobacter sp. strain WH408 | ORF1::lacZ, Kmr | This study |
| Acinetobacter sp. strain WH409 | ORF4::lacZ, Kmr | This study |
| E. coli DH5α | recA1 endA1 supE44 gyrA96 thi hsdR17(rK− mK+) relA1 φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 | 21 |
| Plasmids | ||
| pACYC184 | Cmr Tcr | 20 |
| pBluescript II SK+ | Apr | Stratagene |
| pKOK6.1 | Apr Kmr, promoterless lacZ | 15 |
| pWH767 | Apr | This study |
| pWH773 | Apr Kmr | This study |
| pWH777 | Apr Cmr | This study |
| pWH779 | Apr Kmr | This study |
| pWH782 | Apr | This study |
| pWH785 | Apr | This study |
| pWH786 | Apr | This study |
| pWH787 | Apr | This study |
| pWH788 | Apr | This study |
| pWH789 | Apr | This study |
| pWH790 | Apr | This study |
| pWH791 | Apr Kmr | This study |
| pWH1274 | Apr Tcr, shuttle vector | 12 |