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. 1998 Apr;64(4):1203–1209. doi: 10.1128/aem.64.4.1203-1209.1998

FIG. 1.

FIG. 1

PCR amplification of the gyrB fragments of strains BH and E6. For the upper part of each gel 10-fold serial dilutions of each pure culture were mixed with the activated sludge, and DNA extraction from the mixed samples and PCR amplification of the extracted DNA were carried out. (a) Strain BH. Lane 1, 50-2500 DNA size marker (FMC Corporation); lane 2, 2.5 × 107 cells per ml; lane 3, 2.5 × 106 cells per ml; lane 4, 2.5 × 105 cells per ml; lane 5, 2.5 × 104 cells per ml; lane 6, 2.5 × 103 cells per ml; lane 7, 2.5 × 102 cells per ml; lane 8, 2.5 × 101 cells per ml; lane 9, 2.5 × 100 cells per ml; lane 10, uninoculated activated sludge; lane 11, pure BH culture. (b) Strain E6. Lane 1, 50-2500 DNA marker; lane 2, 1.9 × 107 cells per ml; lane 3, 1.9 × 106 cells per ml; lane 4, 1.9 × 105 cells per ml; lane 5, 1.9 × 104 cells per ml; lane 6, 1.9 × 103 cells per ml; lane 7, 1.9 × 102 cells per ml; lane 8, 1.9 × 101 cells per ml; lane 9, 1.9 × 100 cells per ml; lane 10, uninoculated activated sludge; lane 11, pure E6 culture. For the lower part of each gel DNA was extracted from activated sludge containing strains BH and E6 at densities of 2.5 × 107 and 1.9 × 107 cells per ml, respectively, before 10-fold serial dilution of the extracted DNA with DNA extracted from the uninoculated activated sludge. PCR amplification was carried out by using the diluted samples. (a) Strain BH. (b) Strain E6. Lanes 1, 50-2500 DNA size marker; lanes 2, undiluted DNA; lanes 3, 10-fold dilution; lanes 4, 102-fold dilution; lanes 5, 103-fold dilution; lanes 6, 104-fold dilution; lanes 7, 105-fold dilution; lanes 8, 106-fold dilution; lanes 9, 107-fold dilution; lanes 10, uninoculated activated sludge; lanes 11, pure culture.