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Specificity and sensitivity of the PCR method for detecting the BH population in Ohdaira activated sludge. Lane 1, 50-2500 DNA size marker; lane 2, 3.5 × 106 cells per ml; lane 3, 3.5 × 105 cells per ml; lane 4, 3.5 × 104 cells per ml; lane 5, 3.5 × 103 cells per ml; lane 6, 3.5 × 102 cells per ml; lane 7, 3.5 × 101 cells per ml; lane 8, 3.5 × 100 cells per ml; lane 9, uninoculated activated sludge; lane 10, pure BH culture.
