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. 1998 Apr;64(4):1210–1219. doi: 10.1128/aem.64.4.1210-1219.1998

FIG. 3.

FIG. 3

Effect of mercury added to FSL soil on the prevalence of pEC10-like mercury resistance plasmids, as shown by selective plating and colony probing-PCR, exogenous plasmid isolation, and pEC10-specific MPN PCR of soil DNA. (A) Untreated soil. (B) Mercury-treated soil. (C) Mercury-treated soil with added Enterobacter cloacae BE1 Rpr(pEC10). Abbreviations: B, bulk soil; R, rhizosphere soil; total cfu, total counts on 0.1× TSA; Hg res cfu, mercury-resistant CFU; exog. isol. freq., exogenous isolation frequency; BE1 (pEC10), Enterobacter cloacae BE1(pEC10) CFU. The log CFU per gram of dry soil, target numbers per gram of dry soil, or log exogenous plasmid isolation frequencies (10−12) are shown. For mercury-resistant counts (untreated soil and mercury-treated soil), a < b < c (P < 0.05). For pEC10 targets (untreated soil and mercury-treated soil), d < e. For exogenous isolation frequencies (mercury-treated soil), f < g < h (P < 0.05). For BE1 Rpr(pEC10) counts [mercury-treated soil with added BE1 Rpr(pEC10)], i < j (P < 0.05). For pEC10 isolation frequencies (mercury-treated soil with added BE1), k < l < m (P < 0.05). The asterisk indicates that values were not determined.