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. 2023 Oct 28;12(11):12340. doi: 10.1002/jev2.12340

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© 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Palmitate enhances EV secretion in vitro from cortical neurons and oligodendrocytes. EVs were purified from control (BSA‐treated) (a) HK‐532 neurons or (b) oligodendrocyte culture supernatant by ultracentrifugation. EVs and lysate were analysed by WB for TSG101, flotillin and Alix (EV biomarkers) and calnexin, VDAC or argonaute (cellular biomarkers). (c‐i & c‐ii) Nanoparticle tracking analysis profiles of EVs from two different isolations (sample 1 & sample 2). (c‐iii) Concentration of EVs (30–150 nm) and (c‐iv) Particle size distribution analysis of vesicles from two different isolations. EVs were purified from control (Ctrl)‐ or palmitate (Pal, 150 μM, 48 h)‐treated (d) HK‐532 neurons or (e) oligodendrocyte culture supernatant, and EV and cellular markers were analysed.