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. 2023 Oct 28;12(11):12340. doi: 10.1002/jev2.12340

FIGURE 5.

FIGURE 5

Palmitate and glucose increase EV APP secretion in vitro from cells. (a) Differentiated human HK‐532 cortical stem cells were treated with increasing palmitate or oleate concentrations (0 [BSA vehicle], 50, 75, 100 and 150 μM) for 48 h. EVs were purified from culture supernatant and analysed by WB for APP and flotillin. (b) Relative levels from WB analysis for APP versus flotillin in 0 or 150 μM palmitate or oleate represented in the graph. (c) HK‐532 cells were treated with BSA (control) or 150 μM palmitate for 48 h. EVs were purified from culture supernatant and analysed by WB for CTF (C‐terminal fragment). (d) Relative levels from WB analysis for CTF versus TSG‐101 is represented in the graph. (e) eCNs were treated with control (media) or glucose (glu; 50 mM) for 24 h. EVs were purified from cell culture media by ultracentrifugation and analysed by WB for APP versus flotillin and TSG101 as EV markers. (f) Relative levels from WB analysis for EV APP versus flotillin are represented in the graph. Data are presented as mean ± SEM from at least three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, by Student's t‐test.