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. 2023 Oct 28;14:6883. doi: 10.1038/s41467-023-42661-0

Fig. 7. Inhibition of Exo70, PI4KIIα, or MTM1 leads to re-distribution of PD-L1 to the lysosomes.

Fig. 7

a Confocal imaging of PD-L1 in cells stably expressing control shRNA or shRNAs targeting Exo70, PI4KIIα, or MTM1. LAMP1 is used as a lysosome marker. Zoom-in images of the boxed regions are shown on the right. b Confocal imaging of PD-L1 in cells treated with DMSO, ES2, or PI-273. Zoom-in images of the boxed regions are shown to the right. c Quantification of PD-L1 and LAMP1 double positive vesicles in cells treated with control shRNA or shRNAs targeting Exo70, PI4KIIα, or MTM1 (n = 20 cells). Data are presented as mean ± s.d. P-values are calculated using two-sided unpaired t-test. d Quantification of PD-L1 and LAMP1 double positive vesicles in cells treated with DMSO, ES2, or PI-273 (n = 20 cells). Scale bars are indicated in the panels. Data are presented as mean ± s.d. P-values are calculated using two-sided unpaired t-test.