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. 2023 Oct 28;6:1097. doi: 10.1038/s42003-023-05484-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a A schematic of the method to inject rat RPCs under the renal capsule of neonatal NOD/Shi-scid, IL-2RgKO Jic mice. b, c Immunostaining for rat-specific Kim1 in exogenous rat proximal tubules after 2 weeks, comparing samples without CIS treatment (b) and with CIS treatment (c). d Kim1 positivity of exogenous rat proximal tubule cells (n = 6 sections from 3 biologically independent samples) after CIS treatment. Error bars represent means ± SEM. Data were analyzed using the two-tailed unpaired t-test. ****p < 0.0001. e, f Immunostaining of xenogeneic chimeric nephrons after 2 months showing chimeric glomeruli (Neph+) and proximal tubules (LTL +, white dotted lines). Scale bars, 100 μm in (b) and (c), 500 μm in (e), and 20 μm in (f). CIS, cisplatin; DAPI, 4’,6-diamidino-2-phenylindole; GFP, green fluorescent protein; LTL, lotus tetragonolobus lectin; Neph, Nephrin; Kim1, kidney injury molecule 1; PTC, proximal tubule cell.