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. 2023 Oct 28;6:1097. doi: 10.1038/s42003-023-05484-9

Fig. 8. Human nephrons generated in immunosuppressed neonatal mouse kidneys.

Fig. 8

a A schematic of the protocol followed for inducing nephron progenitor cells (NPCs) from EGFP-labeled human-induced pluripotent stem cells. b Representative images of the flow cytometric analysis of NPCs (n = 12 sections from 3 biologically independent samples). c Microscopic images of the in vitro differentiation of human NPCs for 9 days. d Hematoxylin and eosin-stained section of (c) demonstrating glomeruli (yellow arrows) and tubules formation. e–h Immunostaining of human nephrons in the neonatal kidneys 2 weeks after injection. e A human glomerulus (GFP +, Neph+) supplied by host vessels (yellow arrows. GFP−, CD31+). f CTR1 expression of human tubules (red dotted lines) and host mouse tubules (a yellow dotted line). g, h HuKim1 expression in human proximal tubules (LTL+, yellow arrows), comparing samples without CIS treatment (g) and with CIS treatment (h). Scale bars, 500 μm in (c), 100 μm in (d), 50 μm in (e), (f), (g), and (h). CIS, cisplatin; DAPI, 4’,6-diamidino-2-phenylindole; EGFP, enhanced green fluorescent protein; HuKim1, human-specific kidney injury molecule 1; LTL, lotus tetragonolobus lectin; Neph, Nephrin.