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. 2023 Oct 28;14:6869. doi: 10.1038/s41467-023-42609-4

Fig. 7. Task performance and ALM activity shaped by PPC.

Fig. 7

a Schematic of the optogenetics experiment with VGAT-ChR2-EYFP transgenic mice. Laser was turned on during the delay period in randomly interleaved trials. b Left. Task performance when PPC or vS1 was photoinhibited in normally trained or over-trained mice (PPC: normally trained: **P = 0.005, n = 15 sessions from 5 mice; over-trained: n.s., P = 0.34, n = 12 sessions from 4 mice; vS1: normally trained: P = 0.36, n = 15 sessions from 5 mice; over-trained: n.s., P = 0.23, n = 12 sessions from 4 mice, one-tailed bootstrap). Red crosses indicate mean ± SEM. Right: Summary data of task performance with PPC photoinhibition (Same as left). Error bars indicate mean ± SEM. c Schematic of the designer receptors exclusively activated by designer drugs (DREADDs) experiment and injection site of AAV8-CaMKIIα-hM4D(Gi)-mCherry. d Task performance when PPC was silenced with clozapine N-oxide (**P = 0.009, 18 sessions from 6 mice, one-tailed bootstrap). Red cross indicates mean ± SEM. e Left. Example image of ALM neurons expressing GCaMP6s obtained from (c). Right. Mean ALM choice selectivity in choice-encoding neurons, derived by subtracting left choice activity from right choice activity during the pre-action epoch (**P = 0.008, 16 sessions from 6 mice, one-tailed bootstrap). Red cross indicates mean ± SEM.