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. 2023 Oct 28;14:6894. doi: 10.1038/s41467-023-42229-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c Top, Time-course of actin polymerization under the following conditions: actin alone (black), actin + Arp2/3 complex (light gray), actin + Arp2/3 complex + indicated cortactin constructs (dark gray), actin + Arp2/3 complex + GST-WWCA (magenta), actin + Arp2/3 complex + GST-WWCA + indicated cortactin constructs at increasing concentrations (darker shades represent higher concentrations as indicated). Data are shown as the average curve from three independent experiments with s.d. error bars in lighter color. Bottom, Maximum polymerization rates calculated from the polymerization curves shown on the top (n = 3). Source Data are provided as a Source Data file. The statistical significance of the measurements was calculated using an unpaired, two-tailed t-test (P-values listed in the figure; ns, non-significant). d ITC titrations of cortactin constructs Cort_1-76 (left), Cort_18-76 (middle), and Cort_1-33 (right) into ATP-Arp2/3 complex. The experimental conditions, including temperature (T), protein concentration, and fitting parameters (stoichiometry, N and dissociation constant, K_D) are indicated.