Fig. 2. Genetic ablation of tuft cell reduces gastric adenoma growth.

a Schematic outline of the genetic mouse models used to ablate tuft cells and ILC2s. Tuft cell ablation was achieved in gp130;^F/FTC^Δ mice and compared against gp130;^F/FTC^WT littermate controls. Constitutive ILC2 ablation in gp130;^F/FILC2^Δ mice were compared against gp130;^F/FILC2^WT littermate controls. All cohorts were given two injections of tamoxifen to induce tuft cell ablation at 16-weeks of age. EP = endpoint. b Representative wholemounts of stomachs of gp130^F/F compound mutant mice as described in Fig. 2a. Black dotted circles indicate tumors. Scale bar = 8 mm. c Tumor mass of 17-week-old compound-mutant gp130^F/F mice following genetic tuft cell ablation (gp130;^F/FTC^Δ) compared to Tmx-treated gp130;^F/FTC^WT. N = 7 and 9 respectively. d Tumor mass of 17-week-old ILC2^Δ compared to age matched ILC2^WT. N = 6 and 7 respectively. e Flow-cytometry quantification of SiglecF⁺ CD24⁺ EpCAM⁺ tuft cells in tumors of the indicated genotypes treated as described in Fig. 2a. N = 7, 8, 8 and 9 respectively. f Flow-cytometry quantification of ILC2s as KLRG1⁺CD90.2⁺Lineage^-CD45⁺ in tumors of the indicated genotypes treated as described in Fig. 2a. N = 9, 9, 8 and 9 respectively. g, h Flow-cytometry quantification of iILC2s and nILC2s in tumors of the indicated genotypes treated as described in Fig. 2a. N = 9, 9, 8 and 9 respectively. i Schematic outline of the MNU/NaCl-induced GC mouse model and experimental mouse strains. TC;^ΔILC2^Δ, TC;^WTILC2^Δ, and ILC2;^WTTC^WT mice were treated with MNU for 5 alternating weeks, then were aged for 52 weeks with monthly tamoxifen injections to ablate tuft cells. EP = endpoint. Created with BioRender.com. j Quantification of tumor numbers in MNU/NaCl-treated mice as described in Fig. 2i. N = 10, 5 and 8 respectively. k Kaplan–Meier survival analysis of MNU/NaCl-treated mice, TC;^WTILC2^Δ *p = 0.0175 (cp to TC;^WT ILC2^WT), TC;^ΔILC2^Δ *p = 0.0173 (cp to TC;^WTILC2^WT, Mantel-Cox test). N = 14, 5 and 9 respectively. l Representative images and quantification of relative organoid diameter of 10-day old organoids derived from, gp130;^F/FTC^WT and gp130;^F/FTC^Δ mice. Scale bar = 300μm. n = 288 and 116 respectively. Data represents mean ± SEM, p values from two-sided Student’s t-test or one-way ANOVA and Tukey’s multiple comparisons tests *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns - not significant. Each symbol represents an individual mouse. All Data is from two pooled experiments. Source data and exact p values are provided as a Source Data file.