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. 2023 Oct 28;24:260. doi: 10.1186/s12931-023-02557-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — IL-25 inhibited macrophage M1 polarization in a mouse model of neutrophilia-dominant airway inflammation. (A) Flow cytometry of macrophages in mouse lung tissue. The percentages in the leftmost panel represents the proportion of monocytes, the second panel represents the proportion of living cells, the third panel represents the proportion of CD45⁺ living cells, the fourth panel represents the proportion of F4/80⁺ cells (macrophages). In the rightmost panel, CD45⁺F4/80⁺CD86⁺CD206^- represents M1 macrophage, and CD45⁺F4/80⁺CD86^-CD206⁺ represents M2 macrophage; Representative dot plots showing the percentages of M1 and M2 macrophages in different groups. (B) The proportion of M1 macrophages in lung tissue of mice. (C) The proportion of M2 macrophages in lung tissue of mice. (D-F) Detection of Cd80, inos, Tnfα mRNA level in mouse lung tissue using RT-PCR. (G-I) Detection of Il1β, Ifnγ, and Il17a mRNA level in mouse lung tissue using RT-PCR. There were 4–6 mice in each group. One-way ANOVA was used for statistical analysis (* P < 0.05; ** P < 0.01; *** P < 0.001)