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. 2023 Oct 28;24:260. doi: 10.1186/s12931-023-02557-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — IL-25 inhibited LPS-induced translocation of c-Rel in STAT3-dependent manner in mouse pulmonary macrophages. (A) The level of STAT3 phosphorylation was detected using Western blot. (B) Quantitative analysis of STAT3 phosphorylation in primary culture of mouse pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). (C-D) Western blot was performed to measure the protein levels of c-Rel in the cytoplasm (C) and nucleus (D) of mouse pulmonary macrophages. (E-F) Quantitative analysis of c-Rel expresion in cytoplasm (E) and nucleus (F) of mouse pulmonary macrophages using ImageJ. (G) Representative images of c-Rel immunofluorescence staining in primary culture of macrophage from mouse lung. Scale bar, 50 μm. (H) The protein levels of IL-12 A and IL-23 A of mouse pulmonary macrophages was measured using Western blot. (I-J) Quantitative analysis of IL-12 A and IL-23 A in mouse pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). (K) The graphical abstract of this study, which was drawn by Figdraw. The experiment was repeated 3 times independently, and the similar trend was obtained. One way ANOVA was used for statistical analysis (* P < 0.05; ** P < 0.01; *** P < 0.001)