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. 2023 Oct 16;14:1277120. doi: 10.3389/fimmu.2023.1277120

Figure 2.

Figure 2

Different distribution of ILCs and their related receptors in various layers of normal mouse and human skin. (A) In mice, the subcutaneous and epidermal layers were highly enriched in genes characteristic of ILC2s and ILC3s, respectively, while ILC2s and ILC3s characterized the dermis. Furthermore, mouse skin RNA sequencing studies showed that ILCs in all skin layers expressed Crlf2 (encoding the TSLPR subunit) and Tnfrsf25 (encoding DR3). Dermal ILCs and epidermal ILCs highly express Il18r1 (encoding IL-18R subunit) and Il17rb (encoding IL-25R). Il1rl1 (encoding IL-33R subunit) and Il2ra (encoding IL-2R subunit) were significantly expressed on subcutaneous ILCs. (B) In humans, ILCs are only present in the dermis of normal skin. ILCs in the dermis are mainly composed of ILC1s and ILC3s. Flow cytometry data showed that among all dermal ILCs, ILC1s accounted for approximately 45%, ILC3s for approximately 55%, and ILC2s for approximately 11.6%. However, GATA3+ ILC2 was not detected in skin tissue sections by immunofluorescence. DR3, death receptor 3; IL, interleukin; IL-2R, IL-2 receptor; IL-18R, IL-18 receptor; IL-25R, IL-25 receptor; IL-33R, IL-33 receptor; ILC, innate lymphoid cell; T-bet, T-box transcription factor; TSLPR, thymic stromal lymphopoietin receptor; WT, wild type.