FIG. 1.

¹⁹F NMR spectra of culture medium after 1 h of incubation of whole cells of E. jeanselmei with 2-fluorophenol at 30°C (A), cell extracts from E. jeanselmei incubated for 1 h with 2-fluorophenol at 30°C (B), incubation of purified phenol hydroxylase with 2-fluorophenol (C), and incubation of purified phenol hydroxylase plus purified catechol 1,2-dioxygenase with 2-fluorophenol (D). ¹⁹F NMR chemical shift values were identified as described previously (19) and on the basis of results from the present study (see text and Table 1). The resonance marked “is” is from the internal standard, 4-fluorobenzoate.