TABLE 2.
Partial purification of TCE-RDase
| Purification step | Total protein (mg) | Total activity (μmol of VC/min)a | Sp act (μmol of VC/min/mg of protein) | Yield (%) | Purifi- cation (fold) |
|---|---|---|---|---|---|
| Cell membranes | 292 | 149 | 0.51 | 100 | 1.0 |
| Triton X-100 extract | 87 | 82.4 | 0.95 | 55 | 1.9 |
| HP/M POROS column, step elution | 3.2 | 15.1 | 4.7 | 10 | 9.3 |
| PH/M POROS column, gradient elution | 0.48 | 5.82 | 12.1 | 4 | 24 |
a
TCE-RDase activity was assayed for 10 min by using 5 μmol of cis-DCE as the substrate. No ethene was produced during the assay; therefore, activity is expressed as micromoles of VC produced per minute.