Table 1.
First-Line Anti-TB Drugs Resistant Sub-Lineages and Associated Amino Acid Substitutions /Mutation, Northwest Ethiopia, 2023
| Sub-Lineages | Rifampicin-RIF/R | Isoniazid-INH/H | Ethambutol-EMB/E | Pyrazinamide-PZA/Z | Streptomycine-STR/S | #sub-lineages/Resistant to |
|---|---|---|---|---|---|---|
| rpoB | katG | embB | pncA | rpsL/gibB | ||
| Delhi-CAS | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Met306Ile (atg/atA) | -/ c.386delG | 1/RHE | |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Arg (agc/agA) Ser315Thr (agc/aCc) |
Gln497Arg(cag/cGg) | Val139Gly (gtg/gGg) | -/ c.102delG | 1/RHEZ |
| L4.1.1.1/ X-type | Ser450Leu (tcg/tTg) | - | - | - | -/- | 1/R |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | p.Ser347Ile | -/ p.Gly69As | 1/RHE | |
| L4.2.2.ETH | His445Tyr (cac/Tac) His445Arg (cac/cGc) |
Ser315Thr (agc/aCc) | Met306Ile (atg/atC) | c.66_67insT | -/ p.Gly69Asp | 2/RHEZS |
| Delhi-CAS | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | - | - | -/- | 1/RH |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Gly406Ala (ggc/gCc) | Val180Phe (gtc/Ttc) |
Lys88Thr/ Gly69Asp | 1/RHEZS |
| Delhi-CAS | Leu430Pro (ctg/cCg) | - | - | - | -/- | 1/R |
| L4.3.4.1/LAM | His445Asn (cac/Aac) | Ser315Thr (agc/aCc) | - | - | -/- | 1/RH |
| L4.8/T | Ser431Thr (agc/aCc) | - | - | - | -/- | 1/R |
| L4.1.2.1/Haarlem | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Met306Ile (atg/atA) | - | -/- | 1/RHE |
| L4.2.2.ETH | Gln432Pro Ser441Leu |
Ser315Thr (agc/aCc) | Ser297Ala (tcg/Gcg) | - | -/87delC | 1/RHES |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Arg (agc/agA) Ser315Thr (agc/aCc) |
Gln497Arg(cag/cGg) | Val139Gly (gtg/gGg) | -/102delG | 1/RHEZS |
| Delhi-CAS | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Met306Val(atg/Gtg) | - | Lys43Arg (aag/aGg)/- | 2/RHES |
| L4.2.2.ETH | His445Tyr (cac/Tac) His445Arg (cac/cGc) |
Ser315Thr (agc/aCc) | Met306Ile (atg/atA) | - | -/ p.Gly69Asp | 1/RHES |
| L3.1.1/Delhi-CAS | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | −12C>T Met306Ile (atg/atA) |
- | -/- | 1/RHE |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Met306Ile (atg/atC) | Unknown mutation | -/- | 1/RHEZ |
| L4.2.2.ETH | Ser450Leu (tcg/tTg) | Ser315Thr (agc/aCc) | Met306Ile (atg/atA) Gly406Ser (ggc/Agc) |
Gln141Pro (cag/cCg) | Lys43Arg (aag/aGg)/- | 1/RHEZS |
| Total number of Mtb sub-lineages | 20 | |||||
Notes: R: rifampicin (RIF), H: isoniazid (INH), E: ethambutol (EMB), Z: pyrazinamide (PZA), S: streptomycin (STR), #: number.