Figure 2.

A: Quantification of T cell viability comparing unlabeled primary murine T cells and iron oxide NP labeled T cells after 24 h, 48 h and 7 days of labeling in vitro, as assessed by flow cytometry B: IFN γ secretion of unlabeled vs iron oxide NP labeled T cells after 24 h of labeling, assessed by flow cytometry C: Antigen-specific killing activity of unlabeled vs iron oxide NP labeled murine OT1 T cells co-incubated with Gl261-Ova target cells, assessed by RTCA. The experiment shows the mean of n = 3 independent biological replicates D: LDH release assay measuring antigen-specific killing activity of unlabeled vs iron oxide NP labeled OT-1 T cells co-cultured with Gl261-Ova cells or Gl261 cells