Figure 10.
Dual endogenous stimuli-activatable nanomedicine for cancer theranostics. A) An enzyme/GSH-activatable fluorogenic cisplatin prodrug (P-CyPt) with extracellular self-assembly and intracellular disassembly for FL/PA imaging-guided chemotherapy of liver tumors. a) Schematic illustration of self-assembly and disassembly of the prodrug at different locations. b) Size, c) FL intensity, and d) PA intensity of this fluorogenic prodrug after sequential addition of alkaline phosphatase (ALP) and GSH. e) The IC50 values of the prodrug and its controls on tumor cells (HeLa and HepG-2) and normal cell (HEK-293T). Reproduced with permission 38. Copyright 2021 the Authors, published by Springer Nature. B) A pH/GSH-activatable theranostic nanoprobe (DDASI-NPs) for FL imaging-mediated spatiotemporal monitoring of the chemotherapeutic outcome. a) Scheme of the proposed working principle of the nanoprobe. b) Release profile of DAS at different conditions. c) Semiquantitative analysis of in vivo fluorescence intensity in tumor sites injected with this theranostic nanoprobe. Tumor growth inhibition (TGI) in d) the K562 tumor-bearing mice and e) the H22 tumor-bearing mice after different treatments. Reproduced with permission 185. Copyright 2023, Wiley-VCH.