FIG. 3.

RT-PCR detection of tufA mRNA from E. coli cells (10⁷ cells/ml) killed with 50% (A) or 67% (B) ethanol for 7 min and left at room temperature for 0 min (lanes 2), 10 min (lanes 3), 30 min (lanes 4), 60 min (lanes 5), 120 min (lanes 6), or 16 h (lanes 7). Lanes 1 contain control untreated E. coli sample, and the RT-PCR positive (lane +) and -negative (lane −) controls contain E. coli DNA and sterile water, respectively.