Table 2.
List of histochemical quenching treatments evaluated in the study.
| Reagents | Time | Notes |
|---|---|---|
| 0.3 M Glycine | 30 min | |
| 0.3% (w/v) SBB | 30 min | Sections may dry out during the incubation |
| 0.1% (w/v) NaBH4 | 10 min (3 times) | NaBH4 solution should be ice-cooled |
| 1% (w/v) NaBH4 | 10 min (3 times) | NaBH4 solution should be ice-cooled |
| TrueVIEW® | 5 min | |
| TrueBlack® | 5 min | Sections may dry out during the incubation |