Figure 5. Annotation of dark proteome reveals novel metalloprotease folds.

(A) Structural similarity of putative novel metalloproteases relative to the universe of known enzymes. New predictions are shown in purple, the CSA reference for EC number 3.4.24.83 in green, and other known metalloproteases in yellow. Blue dots are known enzymes in Swissprot, and edges are shown between proteins with similarity of less than 0.001 by Foldseek e-value. (B) Examples of four novel predictions (shown in purple), each aligned with the CSA reference structure (PDB 1PWV; green) using all atoms in the five catalytic residues (His686, Glu687, His690, Tyr728, and Glu735).