Extended Data Fig. 6 |. Effect of different media supplements on growth of PDIM(+) and PDIM(−) Mtb.

a, Growth of PDIM(+) and PDIM(−) Mtb H37Rv in standard 7H9/OADC/glycerol/tyloxapol and b–j, the same media with additional supplements as indicated. k–m, Growth using Tween 80 instead of tyloxapol as the culture detergent. (k) 7H9/OADC/glycerol/Tween 80 and (l) the same media supplemented with 0.1 mM propionate, or (m) 7.4 μM vitamin B₁₂. Mean ± SD for n = 3 biological replicates. Data are representative of at least two independent experiments. (a,b,d,f) show independent experimental repeats for the conditions in Fig. 3b–e. *P < 0.001 for both wt and comp versus ΔppsD; two-way ANOVA with Tukey’s multiple comparison test. For some data points the SD is smaller than the data symbols.