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[Preprint]. 2023 Oct 20:2023.10.20.563165. [Version 1] doi: 10.1101/2023.10.20.563165

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Figure 1: — (A) Experimental strategy for the generation of control, Ucp1-Cre^Evdr hemizygous (1xUcp1-Cre^Evdr) and Ucp1-Cre^Evdr homozygous (2xUcp1-Cre^Evdr) mice.

(B) Expected and observed offspring genotypes obtained from 1xUcp1-Cre^Evdr to 1xUcp1-Cre^Evdr crosses separated by sex. N=251 pups from 46 litters. Statistical significance was calculated using Chi-square test.

(C) Kaplan-Meier survival plot of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr mice from 3 to 6 weeks of age. n= 60 controls, 152 1xUcp1-Cre^Evdr, 23 2xUcp1-Cre^Evdr. Statistical significance was calculated using Log-rank (Mantel-Cox) test.

(D) Representative photographs of alizarin red S stained skulls of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr mice. n=2 females and 2 males.

(E) Skull shape index (ration between condylobasal length and the interorbital constriction length) of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr mice. n=2 females and 2 males.

(F) Body weights of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 7 2xUcp1-Cre^Evdr.

(G) BAT weights of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 5 2xUcp1-Cre^Evdr.

(H) WAT weights of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 5 2xUcp1-Cre^Evdr.

(I) Liver weight of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 5 2xUcp1-Cre^Evdr.

(J) Skeletal muscles weight of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 5 2xUcp1-Cre^Evdr.

(K) Other organs weight of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 7 controls, 9 1xUcp1-Cre^Evdr, 5 2xUcp1-Cre^Evdr.

(L) Representative H&E images of fat depots and liver from control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females. n= 4 per genotype.

Unless otherwise noted, data are mean + SEM and statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001.