Figure 3:

(A) RNA-seq comparing female control and 1xUcp1-Cre^Evdr iBAT gene expression (left). Each dot represents one gene. Corresponding GO analysis (right). Genes and pathways significantly enriched in controls are labeled in orange and those enriched in 1xUcp1-Cre^Evdr are labeled in red.

(B) RNA-seq comparing female control and 1xUcp1-Cre^Evdr psWAT gene expression (left). Each dot represents one gene. Corresponding GO analysis (right). Genes and pathways significantly enriched in controls are labeled in orange and those enriched in 1xUcp1-Cre^Evdr are labeled in red.

(C) RNA-seq comparing female control and 2xUcp1-Cre^Evdr iBAT gene expression (left). Each dot represents one gene. Corresponding GO analysis (right). Genes and pathways significantly enriched in controls are labeled in orange and those enriched in 2xUcp1-Cre^Evdr are labeled in brown.

(D) RNA-seq comparing female control and 2xUcp1-Cre^Evdr psWAT gene expression (left). Each dot represents one gene. Corresponding GO analysis (right). Genes and pathways significantly enriched in controls are labeled in orange and those enriched in 2xUcp1-Cre^Evdr are labeled in brown.

(E) qPCR analysis of iBAT of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females at 6 weeks of age. n= 6.

(F) qPCR analysis of psWAT of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females at 6 weeks of age. n= 6.

(G) qPCR analysis of pgWAT of control, 1xUcp1-Cre^Evdr and 2xUcp1-Cre^Evdr females at 6 weeks of age. n= 6.

(H) Rectal temperature of control and 1xUcp1-Cre^Evdr females undergoing acute cold challenge. n= 3 controls, 4 1xUcp1-Cre^Evdr. Statistical significance was calculated using unpaired t-test within timepoint.

(I) Tail temperature of control and 1xUcp1-Cre^Evdr females undergoing acute cold challenge. n= 3 controls, 4 1xUcp1-Cre^Evdr. Statistical significance was calculated using unpaired t-test within timepoint.

(J) BAT temperature of control and 1xUcp1-Cre^Evdr females undergoing acute cold challenge. n= 3 controls, 4 1xUcp1-Cre^Evdr. Statistical significance was calculated using unpaired t-test within timepoint.

(K) qPCR analysis of iBAT of control and 1xUcp1-Cre^Evdr females after cold challenge or maintained at room temperature. n= 3. Statistical significance was calculated using unpaired t-test between room temperature (RT) and cold samples.

(L) qPCR analysis of psWAT of control and 1xUcp1-Cre^Evdr females after cold challenge or maintained at room temperature. n= 3. Statistical significance was calculated using unpaired t-test between room temperature (RT) and cold samples.

(M) qPCR analysis of pgWAT of control and 1xUcp1-Cre^Evdr females after cold challenge or maintained at room temperature. n= 3. Statistical significance was calculated using unpaired t-test between room temperature (RT) and cold samples.

Unless otherwise noted, data are mean + SEM and statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001. For RNA-seq, differential genes were selected by false discovery rate (FDR) < 0.05 with no fold-change cut-off.