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[Preprint]. 2023 Oct 20:2023.10.20.563165. [Version 1] doi: 10.1101/2023.10.20.563165

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Figure 4: — (A) Expected and observed offspring genotypes obtained from end-point PCR genotyping and FRT copy number assay. n=131 pups. Statistical significance was calculated using Chi-square test.

(B) Copy number assay of FRT. n= 4.

(C) BAT weights of control, Ucp1-fl/+^Ucp1-CreEvdr and Ucp1-fl/fl^Ucp1-CreEvdr males. n= 14 control, 13 Ucp1-fl/+Ucp1-CreEvdr, 9 Ucp1-fl/flUcp1-CreEvdr.

(D) WAT weights of control, Ucp1-fl/+^Ucp1-CreEvdr and Ucp1-fl/fl^Ucp1-CreEvdr males. n= 14 control, 13 Ucp1-fl/+Ucp1-CreEvdr, 9 Ucp1-fl/flUcp1-CreEvdr.

(E) Representative H&E images of fat depots from control, Ucp1-fl/+^Ucp1-CreEvdr and Ucp1-fl/fl^Ucp1-CreEvdr males. n= 4. Scale bar, 50μm.

(F) qPCR analysis of Ucp1 in adipose tissue depots of control, Ucp1-fl/+^Ucp1-CreEvdr and Ucp1-fl/fl^Ucp1-CreEvdr males. Values are relative to those of iBAT control. n= 8.

(G) Western blot of iBAT protein lysates of control, Ucp1-fl/+^Ucp1-CreEvdr and Ucp1-fl/fl^Ucp1-CreEvdr males.

(H) Body of control and Ucp1-fl/fl^{Ucp1-CreERT2Biat} males. n= 7 control, 6 Ucp1-fl/fl^{Ucp1-CreERT2Biat}.

(I) BAT weights of control and Ucp1-fl/fl^{Ucp1-CreERT2Biat} males. n= 7 control, 6 Ucp1-fl/fl^{Ucp1-CreERT2Biat.}

(J) WAT weights of control and Ucp1-fl/fl^{Ucp1-CreERT2Biat} males. n= 7 control, 6 Ucp1-fl/fl^{Ucp1-CreERT2Biat}.

(K) Western blot of iBAT protein lysates of control and Ucp1-fl/fl^{Ucp1-CreERT2Biat} males.

(L) BAT temperature of control and Ucp1-fl/fl^Ucp1-CreEvdr males undergoing acute cold challenge. n= 6 controls, 3 Ucp1-fl/fl^Ucp1-CreEvdr. Statistical significance was calculated using unpaired t-test within timepoint.

(M) Tail temperature of control and Ucp1-fl/fl^Ucp1-CreEvdr males undergoing acute cold challenge. n= 6 controls, 3 Ucp1-fl/fl^Ucp1-CreEvdr. Statistical significance was calculated using unpaired t-test within timepoint.

(N) Rectal temperature of control and Ucp1-fl/fl^Ucp1-CreEvdr males undergoing acute cold challenge. n= 6 controls, 3 Ucp1-fl/fl^Ucp1-CreEvdr. Statistical significance was calculated using unpaired t-test within timepoint.

(O) Rectal temperature of control and Ucp1-fl/fl^{Ucp1-CreERT2Biat} males undergoing acute cold challenge. n= 3 controls, 3 Ucp1-fl/fl^Ucp1-CreEvdr. Statistical significance was calculated using unpaired t-test within timepoint.

Unless otherwise noted, data are mean + SEM. Statistical significance was calculated using unpaired t-test or one-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001.