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The stimulation of SEB promoted activation of Th cells. The enriched naïve T cells were co-cultured with BMDCs pre-stimulation with PBS, OVA (50 μg/ml), SEB (50 ng/ml), and OVA+SEB for 24 h, respectively. And the expression of T-bet, GATA3, and Foxp3 in CD4+ T cells was evaluated. Data were presented as mean ± SEM; statistical significance was determined with one-way ANOVA analysis of variance with Tukey’s multiple comparisons test (*p<.05, ***p<.001, ****p<.0001).
