TABLE 1.
Motility of boar spermatozoa after exposure to cell extracts of Bacillus strains
| Cell extract prepared froma: | % Motile spermatozoab after day:
|
|||
|---|---|---|---|---|
| 1 | 2 | 4 | 5 | |
| Emetic strains | ||||
| B. cereus 4810/72 | <5 | <5 | <5 | <5 |
| B. cereus NC 7401 | <5 | <5 | <5 | <5 |
| Other strains | ||||
| B. cereus ATCC 14579T | >60 | >60 | >60 | >50 |
| B. mycoides ATCC 6462T | >60 | >60 | >60 | >50 |
| B. licheniformis DSM 13T | >60 | >60 | >60 | >50 |
| No bacteria | >60 | >60 | >60 | >50 |
a
Bacillus strains were cultivated for 10 days at 28°C on tryptic soy agar. One hundred mg (wet weight) of cells per ml of distilled water was disrupted by repeated sonication and freezing-thawing and were then filtered (pore size, 0.2 μm).
b
Motility is given as percent spermatozoa expressing rapid motility, as measured by the Hamilton-Thorn sperm analyzer. The average difference between replicate tests was ≤20% when extracts of bacteria toward the sperm cells of three different boars were compared. The sperm suspension, containing 30 × 106 to 50 × 106 spermatozoa per ml, was exposed to extracts from 2 mg (wet weight) of bacteria per ml of extended boar semen.