FIG. 3.
Cyclin B1 associated with 16E1∧E4 is found on the keratins or mitochondria. (A) G1/S-synchronized SiHa cells expressing 16E1∧E4 were fixed at 16 h post-block release. Cells were triple stained for 16E1∧E4 (green), cyclin B1 (red), and keratin 18 (K-18; blue). A DAPI nuclear stain was included (white). The upper panels show a cell prior to keratin collapse, while the lower panels show a cell with collapsed keratin. (B) Saos-2 cells infected with recombinant adenoviruses that express 16E1∧E4 were triple stained for 16E1∧E4 (blue), cyclin B1 (green), and mitochondria (red). A DAPI nuclear stain was included (white). Bar, 10 μm.