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. 2005 Apr;79(7):3998–4011. doi: 10.1128/JVI.79.7.3998-4011.2005

FIG. 5.

FIG. 5.

16E1E4 expression reduces the ability of cyclin B1 to accumulate in the nucleus. G1/S-synchronized SiHa cells expressing β-galactosidase, 16E1E4, or Myt1 were treated with leptomycin B (LMB), beginning 14 h postrelease, and then harvested 2.5 h later. (A) The cells were double stained for β-galactosidase, 16E1E4, or Myt1 (green) and cyclin B1 (red). A DAPI nuclear stain was included (blue). The large white arrow indicates a cell with predominantly cytoplasmic cyclin B1, and the small white arrow indicates a cell in which some of the cyclin B1 has entered the nucleus. (B) Total cell extracts were Western blotted for phosphohistone H3 or phosphokeratin 18 serine 33 and compared to a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control. (C and D) G1/S-synchronized SiHa cells expressing 16E1E4 at 24 h post-block release were immunostained for 16E1E4 (green) and phosphokeratin 18 serine 33. A DAPI nuclear stain was included (blue). Bars, 10 μm.