FIG. 6.
Mutant unable to induce G2 arrest fails to colocalize with cyclin B1 and prevent its nuclear entry. Wild-type 16E1∧E4, mutant T22A,T23A-16E1∧E4, and GFP were expressed in SiHa and Cos-7 cells by transfection, and the localization of cyclin B1 was determined by immunofluorescence microscopy. (A) SiHa cells were double stained for 16E1∧E4 (green) and cyclin B1 (red). A DAPI nuclear stain was included (blue). Bar, 10 μm. (B) The percentages of cells showing cyclin B1 colocalized with 16E1∧E4 and showing some nuclear cyclin B1 were determined. The graphs show the mean values of three replicate experiments ± standard error of the mean.