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. 2023 Aug 5;35(11):4133–4154. doi: 10.1093/plcell/koad214

Figure 5.

Figure 5.

Phenotypic analysis of GhMYBL1 RNAi transgenic cotton. A) RT-qPCR analysis of GhMYBL1 expression in 18 DPA fibers of the GhMYBL1 RNAi transgenic lines (T2 generation) and controls (Null and WT). GhUBI1 was used as an internal control for normalization. B) Comparison of mature fiber length of the GhMYBL1 RNAi transgenic lines and controls (Null and WT). C) Measurement and statistical analysis of mature fiber length of the independent GhMYBL1 RNAi transgenic lines and controls (Null and WT) (n ≥ 50, and at least 20 bolls from 15 plants for each independent line). D) Ultrathin sections of 25 and 30 DPA fibers of the GhMYBL1 RNAi transgenic lines (MRiL4 and MRiL5) and controls (Null and WT) by TEM. Scale bars = 2.0 µm. The rectangle marked at a comparable and magnified position in each fiber cell. Scale bars = 1.0 µm. E) Measurement and statistical analysis of fiber cell wall thickness of the GhMYBL1 RNAi transgenic lines and WT (n ≥ 50, and at least 50 seeds from 15 plants for each line). F) Measurement and statistical analysis of crystalline cellulose content in fibers of the GhMYBL1 RNAi transgenic lines and controls (Null and WT). The bolls in the bough located between the third and fourth internodes of cotton plants were randomly collected for fiber phenotypic analysis, and the mean value and Sd were calculated from 3 biological replicates. Values marked with different letters indicate statistically significant differences (P < 0.05) between each group according to the Tukey HSD multiple range test. MRiL, GhMYBL1 RNAi transgenic cotton lines.