FIG. 2.

Localization of CD8⁺ K^bPB1₇₀₃⁺, CD8⁺ K^bNS2₁₁₄⁺, and CD8⁺ K^bM1₁₂₈⁺ IFN-γ⁺ T cells to the respiratory tract of virus secondarily challenged mice. This is the same experiment shown in Fig. 1. Individual BAL samples were stimulated with 1 μM concentrations of the PB1_703-711 (A), NS2_114-121 (B), and M1_128-135 (C) peptides for 5 h and stained for IFN-γ expression (PepC assay). The numbers of IFN-γ⁺ CD8⁺ T cells were calculated and expressed as means ± standard deviations. Differences from the WT group are indicated by an asterisk (P ≤ 0.05, by Student's t test).