FIG. 5.

Prevalence of IFN-γ⁺ TNF-α⁺ CD8⁺ K^bPB1₇₀₃⁺ and CD8⁺ K^bNS2₁₁₄⁺ T cells in a secondary response. The mice were primed and challenged as described in the legend to Fig. 1. The levels of TNF-α⁺ and IFN-γ⁺ production were determined by intracellular cytokine staining subsequent to peptide stimulation (see legend to Fig. 2), and the IFN-γ⁺ TNF-α⁺/IFN-γ⁺ ratios were determined for the BAL fluid and spleen populations. The results are expressed as means ± standard deviations, and significant differences from the findings for the WT group are indicated by an asterisk (P ≤ 0.05).