FIG. 7.
Prnp+/+ mice immunized with PrP retroparticles in CFA and IFA mount PrP-specific antibodies similar to those found after i.v. injection. Prnp+/+ mice (three individuals) were injected s.c. with PrPD111 retroparticles emulsified in CFA and IFA (A) or injected i.v. with PrPD111 retroparticles devoid of adjuvant (B). PrP-specific binding of IgM (left panels) or IgG (right panels) was determined at the indicated days after immunization by FACS analysis with blood of tg33 transgenic mice overexpressing PrP specifically on T cells.