FIG. 2.

Reversion of transformed NIH 3T3 cells treated with the H/N-Ras inhibitor FTI-277. NIH 3T3 cells transformed with pCMV3ΔGP(HA) [3T3(ΔGP(HA))] and with activated H-ras [3T3(H-ras)] were cloned by limiting dilution. Cells were serum starved for 20 to 24 h and treated with dimethyl sulfoxide (vehicle) (a, c, and e) or 5 μM FTI-277 (b, d, and f). Transformed cells were morphologically reversed by treatment with FTI-277 (b and d). Photographs were taken 7 days (a, b, c, and d) or 4 days (e and f) after treatment with FTI-277. Magnification, ×100 in all panels.