TABLE 6.
Effect of MEK-1 inhibitors on transformation of RK3E cellsa
| Inhibitor or DNA | No. of foci in:
|
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Expt 1 | Expt 2 | Expt 3 | Expt 4 | Expt 5 | Expt 6 | |||||||||||||||
| PD98059 (μM) | 0 | 1 | 10 | 20 | 0 | 1 | 10 | 20 | 0 | 5 | 0 | 5 | ||||||||
| Transfected DNA | ||||||||||||||||||||
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||
| pcDNA3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||
| H-ras | 152 | 56 | 12 | 24 | NTb | NT | NT | NT | 38 | 8 | NT | NT | ||||||||
| pCMV3ΔGP | 672 | 332 | 12 | 12 | 892 | 256 | 8 | 8 | 200 | 52 | 196 | 20 | ||||||||
| U0126 (μM) | ||||||||||||||||||||
| Transfected DNA | 0 | 1 | 5 | 10 | 0 | 1 | 5 | 10 | ||||||||||||
| None | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||
| pcDNA3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||
| H-ras | 60 | 16 | 0 | 0 | NT | NT | NT | NT | ||||||||||||
| pCMV3ΔGP | 396 | 8 | 0 | 0 | 392 | 12 | 0 | 0 | ||||||||||||
a
Effect of MEK-1 inhibitors on RK3E transformation was tested under conditions described in the legend to Table 4. Foci were scored 17 to 19 days posttransfection.
b
NT, not tested.