Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HeLa (human cervical adenocarcinoma) | ATCC | RRID: CVCL_0030 | |
| Cell line (Homo sapiens) | SW480 (human colorectal) | ATCC | RRID:CVCL_0546 | |
| Biological sample (Xenopus laevis male) | Sperm | Xenopus I | 5215 Albino Xenopus laevis Sexually Mature Male/-/-/M | Used for fertilization |
| Biological sample (Xenopus laevis female) | Egg | Xenopus I | 4280 Sexually Mature Female 10.5–11 cm/-/-/F | Used for fertilization |
| Biological sample (CD1 NU/NU nude mouse female) | Charles River Laboratories | Xenograft | ||
| Antibody | Pak1 antibody rabbit polyclonal | Abcam | Cat# ab131522, RRID:AB_11156726 | IF (1:100) |
| Antibody | FAK antibody rabbit polyclonal | Cell Signaling | Cat# 3285, RRID:AB_ 2269034 | IF (1:100) |
| Antibody | Rac-1 antibody rabbit polyclonal | Thermo Fisher | Cat# PA1-091 | IF (1:100) |
| Antibody | CD63 antibody mouse monoclonal | Abcam | Cat# ab59479 RRID:AB_ 940915 |
IF (1:100) |
| Antibody | TES antibody rabbit polyclonal | ATLAS | Cat# HPA018123 RRID:AB_1857900 | IF (1:100) |
| Antibody | GSK3 antibody mouse monoclonal | Abcam | Cat# ab93926, RRID:AB_10563643 | IF (1:100) |
| Antibody | Transferrin receptor (TfR) antibody mouse monoclonal | Thermo Fisher | Cat# 13-6800 | IF (1:100) |
| Antibody | ATP6V0a3 antibody rabbit polyclonal | Novus | Cat# nbp1-89333 RRID:AB_11016312 | IF (1:100) |
| Antibody | GAPDH antibody rabbit monoclonal | Cell Signaling | mAb #2118 | WB (1:1000) |
| Antibody | β-Catenin antibody rabbit polyclonal | Thermo Fisher Scientific | Cat# 71-2700; RRID:AB_ 2533982 | IF (1:100) |
| Antibody | β-Catenin antibody mouse monoclonal | Thermo Fisher Scientific | Cat# MA1-2001; RRID:AB_ 326078 | IF (1:100) |
| Antibody | IgG, HRP-linked antibody (horse anti-mouse polyclonal) | Cell Signaling | Cat# 7076; RRID:AB_330924 | WB (1:2500) |
| Antibody | IgG, HRP-linked antibody (goat anti-mouse polyclonal) | Cell Signaling | Cat# 7074; RRID:AB_2099233 | WB (1:2500) |
| Antibody | IgG H&L (Alexa Fluor 594) preadsorbed (goat anti-mouse polyclonal) | Abcam | Cat# ab150120; RRID:AB_2631447 | IF (1:200) |
| Antibody | IgG H&L (Alexa Fluor 594) preadsorbed (goat anti-rabbit polyclonal) | Abcam | Cat# ab150084; RRID:AB_ 2734147 | IF (1:200) |
| Antibody | IgG H&L (Alexa Fluor 488) preadsorbed (goat anti-mouse polyclonal) | Abcam | Cat# ab150117; RRID:AB_ 2734147 | IF (1:200) |
| Antibody | IgG H&L (Alexa Fluor 488) preadsorbed (ab150081) (goat anti-rabbit polyclonal) | Abcam | Cat# ab150081; RRID:AB_2734747 | IF (1:200) |
| Antibody | IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (goat anti-mouse polyclonal) | Invitrogen | Cat# A-11001; RRID:AB_2534069 | IF (1:200) |
| Antibody | IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (goat anti-rabbit polyclonal) | Invitrogen | Cat# A-11011; RRID:AB_143157 | IF (1:200) |
| Recombinant DNA reagent | Dominant-negative (DN)-GSK3-GFP (Xenopus laevis) (plasmid) | Addgene | RRID:Addgene_29681 | K85R and K86R |
| Recombinant DNA reagent | DN-Rab7 (Homo sapiens) (plasmid) | Addgene | RRID:Addgene_12660 | Dominant-negative plasmid |
| Recombinant DNA reagent | pCS2-mGFP (plasmid) | Addgene | RRID:Addgene_14757 | Membrane bound form of EGFP |
| Recombinant DNA reagent | CD63-RFP (Homo sapiens) (plasmid) | Addgene | RRID:Addgene_62964 | RFP tag |
| Recombinant DNA reagent | xWnt8myc (wnt8a.L Frog) (plasmid) | Addgene | RRID:Addgene_16863 | |
| Recombinant DNA reagent | β-Catenin-activated reporter (BAR) (plasmid) | Addgene | RRID:Addgene_12456 | Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter |
| Recombinant DNA reagent | Renilla reporter (plasmid) | Addgene | RRID:Addgene_62186 | MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module |
| Recombinant DNA reagent | Lipofectamine 3000 | Thermo Fisher | Cat# L3000001 | |
| Peptide, recombinant protein | Wnt3a | Peprotech | Cat# 315-20 | |
| Commercial assay or kit | mMESSAGE mMACHINE SP6 Transcription Kit | Thermo Fisher | Cat# AM1340 | |
| Commercial assay or kit | Dual-Luciferase Reporter Assay System | Promega | Cat# E1500 | |
| Chemical compound, drug | Fibronectin | Thermo Fisher | Cat# 33016015 | |
| Chemical compound, drug | Dextran Tetramethylrhodamine (TMR-Dx) 70,000 | Thermo Fisher | Cat# D1818 | |
| Chemical compound, drug | 5-(N-Ethyl-N-isopropyl) amiloride (EIPA) | Sigma | Cat# A3085 | |
| Chemical compound, drug | Bafilomycin A1 | Selleckchem | Cat# S1413 | |
| Chemical compound, drug | Digitonin | Sigma | Cat# 300410 | |
| Chemical compound, drug | Phalloidin | Abcam | Cat# ab176759 | |
| Chemical compound, drug | Lithium chloride (LiCl) | Sigma | Cat# L4408 | |
| Chemical compound, drug | PF-00562271 (FAK inhibitor) | Selleckchem | Cat# S2672 | |
| Chemical compound, drug | Phorbol 12-myristate 13-acetate (PMA) | TOCRIS | Cat# 1201 | |
| Chemical compound, drug | 5-(N-Ethyl-N-isopropyl) amiloride (EIPA) | Sigma | Cat# A3085 | |
| Chemical compound, drug | EHT 1864 | Selleckchem | Catalog# S7482 | |
| Chemical compound, drug | Triton X-100 | Thermo Fisher | Cat# HFH10 | |
| Chemical compound, drug | Paraformaldehyde | Sigma | Cat# P6148 | |
| Chemical compound, drug | Fibronectin | Sigma | Cat# F4759 | |
| Chemical compound, drug | Protease inhibitors | Roche | Cat# 04693132001 | |
| Chemical compound, drug | Phosphatase inhibitors | Calbiochem | Cat# 524629 | |
| Software, algorithm | ImageJ | NIH | http://imagej.nih.gov/ij/ | |
| Software, algorithm | AxioVision 4.8 | Zeiss | http://Zeiss.com | |
| Software, algorithm | Zen 2.3 imaging software | Zeiss | http://Zeiss.com | |
| Software, algorithm | R | R Core Team | https://cran.r-project.org | |
| Other | 10 cm dish | Thermo Fisher | Cat# 174903 | |
| Other | 8-Well glass-bottom chamber slides | ibidi | Cat# 80827 | |
| Other | Circular coverslips | ibidi | Cat# 10815 | |
| Other | 12-Well dish | Thermo Fisher | Cat# 150628 | |
| Other | DMEM (culture medium) | Thermo Fisher | Cat# 11965092 | |
| Other | L-15 (culture medium) | Thermo Fisher | Cat# 11415064 | |
| Other | Glutamine | Thermo Fisher | Cat# 25030081 | |
| Other | Fetal bovine serum (FBS) | Thermo Fisher | Cat# 16000044 | |
| Other | Bovine serum albumin (BSA) | Thermo Fisher | Cat# 9048468 | |
| Other | Pen-Strep antibiotics | Thermo Fisher | Cat# 15140122 | |
| Other | PBS | Gibco | Cat# 10-010-023 | |
| Other | PBS | Fisher Scientific | Cat# BP3994 | |
| Other | Fluoroshield Mounting Medium with DAPI | Abcam | Cat# ab104139 | |
| Other | IM 300 microinjection pump | Narishige International USA, Inc | N/A | |
| Other | Axio Observer Z1 Inverted Microscope with Apotome | Zeiss | N/A |