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Effect of GFP-EH21 on plaque formation. BS-C-1 cells were infected with vaccinia virus vEH21i with or without IPTG, WR, IHD-J, vWEH21, or vIEH21 as indicated. The cells were covered with a semisolid methylcellulose overlay and incubated for 3 days (rows 1, 2, and 3) or with a liquid overlay and incubated for 1 day (bottom row). Plaques were visualized by staining with crystal violet in 20% ethanol.
