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Effect of GFP-EH21 on virus spread. BS-C-1 cells were infected with 0.001 PFU per cell of vEH21i in the absence (−) or presence (+) of IPTG, of vWEH21 or vWGFP control, or of vIEH21 or vIGFP control. Cell monolayers were harvested at 24-h intervals. Cells were lysed, and virus titers were determined by plaque assay. The data presented are means, with the standard deviations, of three separate infections.
