FIG. 2.

CpG ODN2216 increases monocyte expression of CD80, CD86, CD40, and HLA-DR. (A) Representative flow histograms from a healthy donor and an HIV+ donor. Filled histograms represent surface molecule expression in cells cultured 24 h without stimuli; solid lines represent surface molecule expression in cells cultured with CpG ODN2216 (3 μg/ml) for 24 h. (B) Summary results of all studies in HIV− and HIV+ donors. Statistically significant differences in the expression of CD80, CD86, CD40, and HLA-DR surface expression among cells incubated in medium alone versus cells incubated with CpG ODN2216 are shown (P values determined by two-tailed nonpaired Student's t test). The numbers shown above the data points are the mean values for the respective observations.