FIG. 6.

CPG ODN2216 alters monocyte surface phenotype indirectly via soluble factor(s). Monocytes were isolated from PBMC by magnetic bead selection. Purified monocytes were then incubated with CpG ODN or IFN-α. Some purified monocytes were incubated in Transwells with PBMC in communicating wells ± CpG ODN. Induction of CD40 and CD80 expression was examined with purified monocytes (top), among monocytes within PBMC (middle), and with purified monocytes suspended in transwells with PBMC in communicating wells (bottom) following incubation in medium alone (filled histograms) or in medium supplemented with CpG ODN (solid lines) or IFN-α (1,000 U/ml; dashed lines). The experiment shown is representative of the three experiments performed.