FIG. 7.

Blockade of the IFN-α/β receptor interferes with CpG ODN2216 or IFN-α-induced monocyte maturation. PBMC were cultured in medium alone or in medium supplemented with CpG ODN2216 (3 μg/ml) or IFN-α (10,000 U/ml). Murine antibody to the human IFN-α/β receptor (anti-IFNa/bR; 10 μg/ml) or isotype control antibody (10 μg/ml) was added at the beginning of culture as indicated. MFI (y axis) of CD80 and CD40 staining among CD14⁺ monocytes as determined by flow cytometric analysis is shown. These results are the mean values for four experiments; P values were determined by paired t test. Although not depicted, statistical differences were observed between cells cultured in medium alone and cells cultured with either CpG ODN or IFN-α (P < 0.05). Ig, immunoglobulin.