FIG. 2.

Southern hybridization to identify genomic regions on clones of PsADH1 and PsADH2. (A) Approximate location of the PsADH1 coding region on the 6.0-kbp BamHI-XbaI PsADH1 DNA fragment as determined by restriction mapping and cross-hybridizations of the resulting restriction fragments to ScADH1. +, positive hybridization; −, no hybridization. Bold lines represent the fragment containing 5′- and 3′-flanking regions of PsADH genes. (B) Approximate location of the PsADH2 coding region on the 6.6-kbp BamHI-SalI PsADH2 DNA fragment as determined by nested deletions from the 5′ end and cross-hybridizations of the resulting fragments to ScADH1. +, positive hybridization; −, no hybridization. The boxes indicate the PsADH1 (A) and PsADH2 (B) coding regions.