FIG. 7.

Aerobic growth of P. stipitis wild-type CBS 6054, parental, and Psadh disruptant strains on either ethanol (A) or glycerol (B). Cells were grown in YNB with either 2% ethanol or 3% glycerol plus 100 μg of leucine or uridine/ml, as needed, with shaking in a 125-ml baffled flask at 180 rpm and 30°C. Symbols: ▪, CBS 6054 (wild type); •, PSU1 (ura3-2/ura3-2); ▴, PLU20 (ura3-3/ura303 Psleu2Δ-1/Psleu2Δ-1); ▵, PLU123 (Psadh2 disruptant); ○, PSU218 (Psadh1 disruptant); □, PLU1209 (Psadh double disruptant).