Figure 7.

Myeloid cell subclusters. (A) UMAP plots of all myeloid cell subclusters (NK, DC; left panel) divided by healthy controls and patients (right panel). (B) Heatmap of the expression of marker genes used to identify the cell type of each subcluster. Red and blue colors denote upregulated and downregulated genes, respectively. (C) Trajectory plot describing the degree of differentiation and subcluster distribution by pseudotime analysis. The arrow represents the direction of differentiation. (D) Proportion of myeloid cell subclusters (left) and number of cells (right) in each sample. (E) Box-and-whisker plot comparing the proportion of myeloid cell subclusters 3, 4, and 7 between healthy controls and patients. The solid horizontal line represents the median, the lower boundary represents the lower quartile, the upper border represents the upper quartile, and the whiskers represent outliers (t-test, *P < 0.05). (F) Volcano plots of DEGs of myeloid cell subclusters 3, 4, and 7 compared with the other clusters. Red and blue dots denote upregulated and downregulated DEGs, respectively, with an adjusted P value <0.05 and a |log₂(FC: fold change)| >0.25. AA = aplastic anemia; DC = dendritic cell; DEG = differentially expressed genes; NK = natural killer cell; UMAP = uniform manifold approximation and projection.