TABLE 2.
Real-time RT-PCR detection of β-actin, LMP-1, and LMP2A
| Samplea | Transcript | CTc | Input | Avg input ± SD | Fold inductionb |
|---|---|---|---|---|---|
| EBNA2 | Actin | 20.40 | 0.1892 | 0.2005 ± 0.0160 | |
| EBNA2 | Actin | 20.22 | 0.2119 | ||
| EBNA2 + LP | Actin | 20.31 | 0.2002 | 0.2074 ± 0.0101 | |
| EBNA2 + LP | Actin | 20.20 | 0.2146 | ||
| EBNA2 | LMP-1 | 26.28 | 4.9671 | 5.1025 ± 0.1909 | 1.000 ± 0.0883 |
| EBNA2 | LMP-1 | 26.17 | 5.2372 | ||
| EBNA2 + LP | LMP-1 | 22.76 | 26.9153 | 27.111 ± 0.2781 | 5.138 ± 0.0501 |
| EBNA2 + LP | LMP-1 | 22.73 | 27.3086 | ||
| EBNA2 | LMP2A | 31.81 | 6.7019 | 6.6091 ± 0.1312 | 1.000 ± 0.0824 |
| EBNA2 | LMP2A | 31.85 | 6.5163 | ||
| EBNA2 + LP | LMP2A | 31.67 | 7.3978 | 7.5569 ± 0.2250 | 1.105 ± 0.0574 |
| EBNA2 + LP | LMP2A | 31.61 | 7.7161 |
a
RNA from cells transfected with the indicated plasmids was reverse transcribed, and the cDNA was amplified by real-time PCR as described in Materials and Methods.
b
Relative (n-fold) induction was calculated by using the AB User Bulletin 2 calibrator method for relative quantitation; relative transcript induction was normalized to that of actin and then expressed as a ratio to the EBNA2-only sample.
c
CT, number of PCR cycles at the threshold.