FIG. 2.

Immunoblot assay of glut-1 in MDBK cells. MDBK cells were lysed, and proteins were separated by SDS-PAGE, blotted onto nitrocellulose, and probed with rabbit anti-glut-1 antiserum, followed by goat anti-rabbit antiserum conjugated to horseradish peroxidase. Lanes: 1, MDBK.cycT1 cells; 2, MDBK.empty cells; 3, MDBK.glut-1 cells; 4, MDBK.cycT1.empty cells; 5, MDBK.cycT1.glut-1 cells. Note the heterogeneous reactivity at 55 kDa; other bands are presumably background or perhaps reflect endogenous glut-1 reactivity.